Activated Charcoal—A Potential Material in Glucoamylase Recovery

نویسندگان

  • S. O. Kareem
  • I. Akpan
  • T. O. S. Popoola
  • L. O. Sanni
چکیده

The potential of activated charcoal in the purification of fungal glucoamylase was investigated. Various concentrations of activated charcoal (1-4% w/v) were used to concentrate crude glucoamylase from Rhizopus oligosporus at different temperature values (30-50°C). Effects of pH (3.0-6.0) and contact time (0-60 min) on enzyme purification were also monitored. Activated charcoal (3% w/v) gave a 16-fold purification in a single-step purification at 50°C for 20 min and pH 5.5. The result of SDS-PAGE analysis of purified glucoamylase showed two major protein bands with corresponding molecular weight of 36 kDa and 50 kDa. The method is inexpensive, rapid, and simple which could facilitate downstream processing of industrial enzyme.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Comparison of Deferoxamine, Activated Charcoal, and Vitamin C in ‎Changing the Serum Level of Fe in Iron Overloaded Rats

Background: Iron is an essential mineral for normal cellular physiology but its ‎overload can lead to cell injury. For many years, deferoxamine injection has been used ‎as an iron chelator for treatment of iron overload. The aim of this study is to compare ‎oral deferoxamine, activated charcoal, and vitamin C, as an absorbent factor of Fe, in ‎changing the serum level of iron in iron overload r...

متن کامل

اثرات اصلاح اسیدی کربن فعال در جذب سطحی گاز بیهوشی سووفلوران

Introduction: Anesthetic gases used in hospitals include N2O and halogenated hydrocarbons (such as sevoflurane) which can be released through leakages and uncontrolled exhalation by the patient into the ambient air of clinical staff. These gases have greenhouse effect and damage to the ozone layer and serious risks such as reproductive, preterm delivery and fetal abnormalities and increased spo...

متن کامل

استفاده از ریزنمونه‏های گره و ترکیبات مختلف تنظیم‌کننده‏های رشد در باززایی مستقیم پروانش ((Catharanthus roeus L.

Periwinkle (Catharanthus roseus L., Apocynaceae) contains more than 130 different terpenoid indole alkaloids (TIAs), of which two dimeric alkaloids, Vinblastine and Vincristine, have antineoplastic activity and are useful in treatment of various cancers. Specific production of some alkaloids in differentiated tissues such as leaf and stem led to use direct regeneration of explants in order to i...

متن کامل

Xylene removal from dilute solution by palm kernel activated charcoal: Kinetics and equilibrium analysis

Xylene is an aromatic hydrocarbon that is a highly toxic compound. Therefore, it is essential to remove this component from wastewater before discharging it to the environment. In this research work, palm kernel biomass was activated chemically by H3PO4 and synthesized activated charcoal was applied to separate xylene from aqueous media. The prepared activated charcoal was characterized using F...

متن کامل

Nitrate Removal from Aqueous Solutions Using Almond Charcoal Activated with Zinc Chloride

Background & Aims of the Study: Nitrate is one of the most important contaminants in aquatic environments that can leached to water resources from various sources such as sewage, fertilizers and decomposition of organic waste. Reduction of nitrate to nitrite in infant’s blood stream can cause “blue baby” disease in infants. The aim of this study was to evaluate the...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:

دوره 2011  شماره 

صفحات  -

تاریخ انتشار 2011